William Murphy, Ph.D.'s picture
William
Murphy, Ph.D.

University of Wisconsin-Madison

Title of Abstract

Assembly of Stem Cell-derived Human Tissues for Screening Applications

Presenting Author

William L. Murphy

Abstract

The need for human, organotypic culture models coupled with the requirements of contemporary drug discovery and toxin screening (i.e. reproducibility, high throughput, transferability of data, clear mechanisms of action) frame an opportunity for a paradigm shift. The next generation of high throughput cell-based assay formats will require a broadly applicable set of tools for human tissue assembly and analysis. Toward that end, we have recently focused on: i) generating iPS-derived cells that properly represent the diverse phenotypic characteristics of developing or mature human somatic cells; ii) assembling organotypic cell culture systems that are robust and reproducible; iii) translating organotypic cell culture models to microscale systems for high throughput screening; and iv) combining genomic analyses with bioinformatics to gain insights into organotypic model assembly and the pathways influenced by drugs and toxins. This talk will emphasize recent studies in which we have explored biologically driven assembly of 3-dimensional “cellular scaffolds” and organotypic vascular, neural, and hepatic tissues. These tissues mimic critical aspects of human organs, and can be used for reproducible identification of toxic compounds.

Research Interests

In the Bioinspired Materials Lab (BML), we are building innovative biomaterials to address significant challenges in biology and medicine. We bring together chemists, biologists, materials scientists, and engineers to create new materials we refer to as "bioinspired materials". Our materials are "bioinspired" because they mimic some of the fascinating ways that nature builds materials – from sea shells to human organs. We work closely with biologists to make fundamental scientific discoveries. We also partner with clinicians to create medical devices that can regenerate diseased or damaged tissues. We are driven to achieve high impact by pursuing groundbreaking discoveries, translating discoveries into new medical treatments, and training the next generation of leaders in regenerative medicine.

Author Listing

William L. Murphy

Affiliation

University of Wisconsin-Madison

All Author Affiliations

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Stem Cell and Regenerative Medicine Center, University of Wisconsin, Madison, WI